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Protein Mass Spectrometery, 3 credits

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The course syllabus was confirmed by the Faculty board for graduate studies 25 November 2016. The course is in the third cycle and amounts to 3 credits.

Learning outcomes

On completion of the course, participants shall be able to:

Knowledge and understanding

Describe the principles of mass spectrometry, including the basic design of the instrument and its operation.
Describe the most common mass spectrometry applications, including possibilities and limitations for analysis.
Plan and execute the best suited sample preparations and mass spectrometry acquisition.

Skills and abilities

Make an experimental plan for preparing and analyzing protein and peptide samples using mass spectrometry.
Set up and perform experimental work including purification, concentration and separation of samples.
Carry out a mass spectrometry analysis and handle the aquired data using common mass spectrometry software.

Judgement and approach

Plan for optimal sample preparations and analysis depending on sample type and aim of the analysis.
Explain which types of results that can be obtained with mass spectrometry.
Compare results from different analyses and from an analytical point of view explain differences and similarities.

Course content

The course gives an introduction in how to use protein mass spectrometry as a tool in protein science. Practical parts including protein and peptide sample preparations, deposition with different techniques and the use of chromatographic separations for complex samples prior to mass spectrometry analysis. Manual and automated mass spectrometry acquisition of intact protein and peptide mass determination and determination of peptide sequence information. Basic data handling of mass spectrometry data for protein identification, assessment of PTMs and determination of amino acid sequences.

Teaching

The course consists of one intense week of seminars and experimental work. Projects are performed in groups of 1-4 participants (work in the lab, analysis by the mass spectrometer and data analysis). Time is allocated for independent projects with the goal to develop own interest and possibility to get feedback on own ideas/experimental strategies.

Assessment

Assessment is based on attendance, written assignments, and oral presentation.

Grading scale

Possible grades are Pass and Fail. To pass the course, the student must have (1) >95% attendance to all lectures and practicals, (2) handed in written reports and (3) made an oral presentation summarizing the literature study.

Language of instruction

English.

Entry requirements

Basic knowledge in protein chemistry.

Course responsible teacher and unit/division

Katja Bernfur
Email: katja.bernfur@biochemistry.lu.se

Centre for Molecular Protein Science (CMPS), KILU

Do you have questions? Please contact viveka.alfredsson@chem.lu.se