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Bioanalytical HPLC, 3 credits

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The course syllabus was confirmed by the Faculty board for graduate studies 30 June 2016. The course is in the third cycle and amounts to 3 credits.
The course syllabus is formally approved in Swedish. This is a translation.

Learning outcomes

On completion of the course, participants shall be able to:

Knowledge and understanding

• provide advanced explanations for liquid chromatographic principles relevant for bioanalytical applications
• describe the construction and function of pumps and detectors for liquid chromatographic analysis
• explain the principles of various extraction methods and highlight the importance of proper sample preparation prior to liquid chromatographic analysis

Skills and abilities

• evaluate the choice of liquid chromatographic separation method based on analyte polarity, charge and size
• apply optimization strategies with respect to retention and selectivity for quantitative as well as qualitative analysis

Course content

Lectures: Advanced theoretical treatment of liquid chromatographic separation and the underlying distribution and adsorption equilibria. Instrumentation and experimental technology for high-performance liquid chromatography and universal and selective detectors. Coupling high-performance liquid chromatography to high resolution mass spectrometry. Sample preparation of biological samples prior to liquid chromatographic analysis.
Laboratory work: Experimental technology for high-performance liquid chromatography. Optimisation of HPLC systems with different combinations of mobile phases for quantitative and qualitative analysis of small molecules. Demonstration of high-performance liquid chromatography in combination with high-resolution mass spectrometry for qualitative analysis of small molecules.

Teaching

Preparation through literature studies prior to course start is required. The course is instructed mostly through lectures, also some exercises for self-studies are provided. A demonstration of LC-MS and one day of independent laboratory work is included. The course ends with an oral seminar.

Assessment

Assessment is based on attendance and on the oral seminar

Grading scale

Possible grades are Pass and Fail. To pass the course, the student must attend all components and pass the oral seminar.

Language of instruction

The course is given in English.

Entry requirements

None

Course responsible teacher and unit/division

Margareta Sandahl
Email: margareta.sandahl@chem.lu.se

Centre for Analysis and Synthesis (CAS), KILU

Do you have questions? Please contact FUstudierektor@kilu.lu.se